Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis


Gurakan G. C. , Bayindirli A. , Ogel Z., Tanboga P.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, cilt.14, ss.293-295, 1998 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 14 Konu: 2
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1023/a:1008815120601
  • Dergi Adı: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Sayfa Sayıları: ss.293-295

Özet

Plasmid pSB6 is a streptococcal recombinant plasmid carrying the alpha-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher levels of structural instability under the conditions examined.