Stability of a recombinant plasmid carrying alpha-amylase gene in Bacillus subtilis
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.14, no.2, pp.293-295, 1998 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 14 Issue: 2
- Publication Date: 1998
- Doi Number: 10.1023/a:1008815120601
- Journal Name: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.293-295
- Middle East Technical University Affiliated: Yes
Abstract
Plasmid pSB6 is a streptococcal recombinant plasmid carrying the alpha-amylase gene of Bacillus amyloliquefaciens and the chloramphenicol resistance gene. The segregational and structural instabilities of this plasmid were examined under non-selective conditions in Bacillus subtilis. These instabilities were modelled according to a kinetic expression derived from the difference in the growth between plasmid-bearing and plasmid-free cells. This plasmid showed slight segregational instability and much higher levels of structural instability under the conditions examined.