Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor


Marchenko S. V. , Soldatkin O. O. , Kasap B. O. , AKATA KURÇ B., Soldatkin A. P. , Dzyadevych S. V.

NANOSCALE RESEARCH LETTERS, vol.11, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 11
  • Publication Date: 2016
  • Doi Number: 10.1186/s11671-016-1386-9
  • Journal Name: NANOSCALE RESEARCH LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Biosensor, Creatinine, Silicalite, pH-sensitive field-effect transistor, FIELD-EFFECT TRANSISTORS, IMMOBILIZED UREASE, ENZYME ADSORPTION, ZEOLITE-BETA, IMPROVEMENT, CRYSTALS, SENSOR
  • Middle East Technical University Affiliated: Yes

Abstract

In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehycie vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two-to threefold increased sensitivity to creatinine, three-to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 mu m, etc.).