Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor
NANOSCALE RESEARCH LETTERS, vol.11, 2016 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 11
- Publication Date: 2016
- Doi Number: 10.1186/s11671-016-1386-9
- Journal Name: NANOSCALE RESEARCH LETTERS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Keywords: Biosensor, Creatinine, Silicalite, pH-sensitive field-effect transistor, FIELD-EFFECT TRANSISTORS, IMMOBILIZED UREASE, ENZYME ADSORPTION, ZEOLITE-BETA, IMPROVEMENT, CRYSTALS, SENSOR
- Middle East Technical University Affiliated: Yes
Abstract
In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehycie vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two-to threefold increased sensitivity to creatinine, three-to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 mu m, etc.).