Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor

Marchenko S. V. , Soldatkin O. O. , Kasap B. O. , AKATA KURÇ B. , Soldatkin A. P. , Dzyadevych S. V.

NANOSCALE RESEARCH LETTERS, cilt.11, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1186/s11671-016-1386-9


In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehycie vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two-to threefold increased sensitivity to creatinine, three-to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 mu m, etc.).