Transcriptome analysis of Rhodobacter capsulatus grown on different nitrogen sources

Erkal N. A., Eser M. G., Ozgur E., GÜNDÜZ U., EROĞLU İ., Yucel M.

ARCHIVES OF MICROBIOLOGY, cilt.201, sa.5, ss.661-671, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 201 Sayı: 5
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s00203-019-01635-x
  • Dergi Adı: ARCHIVES OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.661-671
  • Anahtar Kelimeler: Photoheterotrophic growth, Rhodobacter capsulatus, Purple non-sulfur bacteria, Microarray, Hydrogen metabolism, Acetate metabolism, HYDROGEN-PRODUCTION, PHOTOHETEROTROPHIC GROWTH, RHODOSPIRILLUM-RUBRUM, ACETATE ASSIMILATION, COMPLEX I, METABOLISM, LYASE, PATHWAYS, FIXATION, BACTERIA
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

This study investigated the effect of different nitrogen sources, namely, ammonium chloride and glutamate, on photoheterotrophic metabolism of Rhodobacter capsulatus grown on acetate as the carbon source. Genes that were significantly differentially expressed according to Affymetrix microarray data were categorized into Clusters of Orthologous Groups functional categories and those in acetate assimilation, hydrogen production, and photosynthetic electron transport pathways were analyzed in detail. Genes related to hydrogen production metabolism were significantly downregulated in cultures grown on ammonium chloride when compared to those grown on glutamate. In contrast, photosynthetic electron transport and acetate assimilation pathway genes were upregulated. In detail, aceA encoding isocitrate lyase, a unique enzyme of the glyoxylate cycle and ccrA encoding the rate limiting crotonyl-CoA carboxylase/reductase enzyme of ethylmalonyl-coA pathway were significantly upregulated. Our findings indicate for the first time that R. capsulatus can operate both glyoxylate and ethylmalonyl-coA cycles for acetate assimilation.