Comparison of antioxidant enzyme activities and DNA damage in chickpea (Cicer arietinum L.) genotypes exposed to vanadium

Imtiaz M., Mushtaq M. A., Rizwan M. S., Arif M. S., Yousaf B., Ashraf M., ...More

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol.23, no.19, pp.19787-19796, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 19
  • Publication Date: 2016
  • Doi Number: 10.1007/s11356-016-7192-1
  • Journal Name: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.19787-19796
  • Keywords: Vanadium, Chickpea genotypes, DNA damage, Ion leakage, Photosynthetic pigments, PROGRAMMED CELL-DEATH, CHINESE-CABBAGE, HEAVY-METALS, CADMIUM, ENDONUCLEASE, RESPONSES, DEFENSE, STRESS, PLANTS, RISK
  • Middle East Technical University Affiliated: No

Abstract

The present study was done to elucidate the effects of vanadium (V) on photosynthetic pigments, membrane damage, antioxidant enzymes, protein, and deoxyribonucleic acid (DNA) integrity in the following chickpea genotypes: C-44 (tolerant) and Balkasar (sensitive). Changes in these parameters were strikingly dependent on levels of V, at 60 and 120 mg V L-1 induced DNA damage in Balkasar only, while photosynthetic pigments and protein were decreased from 15 to 120 mg V L-1 and membrane was also damaged. It was shown that photosynthetic pigments and protein production declined from 15 to 120 mg V L-1 and the membrane was also damaged, while DNA damage was not observed at any level of V stress in C-44. Moreover, the antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were increased in both genotypes of chickpea against V stress; however, more activities were observed in C-44 than Balkasar. The results suggest that DNA damage in sensitive genotypes can be triggered due to exposure of higher vanadium.