Akademik Veri Yönetim Sistemi
THEORETICAL AND APPLIED GENETICS, cilt.139, sa.1, 2025 (SCI-Expanded, Scopus)
Key messageNet-form net blotch (NFNB) is a devastating fungal disease for barley. Three potentially novel QTL for resistance and identified SNP markers can contribute to the global control efforts of NFNB.AbstractPyrenophora teres f. teres, the fungus responsible for the barley disease, net-form net blotch (NFNB), leads to considerable yield and quality reductions. This research involved collecting phenotypic and genotypic data from a barley doubled haploid (DH) mapping population consisting of 277 lines, which were exposed to the highly virulent Ptt isolate GPS18. The DH lines were derived via anther culture from second-generation hybrids of a cross between the disease-resistant barley cultivar Avc & imath; 2002 ("A") and the susceptible cultivar B & uuml;lb & uuml;l 89 ("B"). Anther pretreatment with 1.0 M mannitol resulted in a statistically superior response compared to 0.7 M mannitol in the F2 progeny of the A x B cross. The highest callus induction rate was 37.6% in the "Br_Ind" medium, and the highest green plant formation rate was 24.7% in the "PD_Reg" medium. The use of sequencing-based diversity array technology (DArT-seq) identified 9170 SNP markers, which facilitated the creation of a linkage map spanning 1682.97 cM, with an average density of 1.49 markers/cM. Quantitative trait loci (QTL) analysis identified three QTL associated with Ptt resistance located on chromosomes 3H, 4H, and 6H. All three can be considered novel with the 3H QTL mapping in between Rpt1 and QRptta3, the 4H QTL maps to a distinct region of Rpt7, and the 6H QTL maps in between the Qns-6H.3 and SFNB-6H-33.74 loci. The SNPs associated with disease resistance identified within these QTL offer a foundation for developing DNA-based tests for resistance.