Determination of Selected Endocrine Disrupter Compounds at Trace Levels in Sewage Sludge Samples

Sonmez M. S. , Muz M., Komesli O. T. , BAKIRDERE S., Gokcay C. F.

CLEAN-SOIL AIR WATER, vol.40, no.9, pp.980-985, 2012 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 9
  • Publication Date: 2012
  • Doi Number: 10.1002/clen.201100403
  • Title of Journal : CLEAN-SOIL AIR WATER
  • Page Numbers: pp.980-985


Simultaneous determination of endocrine disrupter compounds (EDCs), namely diltiazem, progesterone, benzylbutylphthalate (BBP), estrone, and carbamazepine (Cbz) were performed by using high performance LC-electrospray tandem MS. The ultrasound-aided sequential extraction of sludge samples was optimized to increase extraction efficiencies of the analytes; ranging between 93.0-98.3% recovery. The limit of detection values for diltiazem, progesterone, BBP, estrone, and Cbz were found as 0.78, 0.72, 0.24, 0.75, and 0.72 mu g/kg, respectively. Sludge samples taken from Ankara Tatlar; Hurma, Lara and Kemer of Antalya, and Middle East Technical University-vacuum rotating membrane wastewater treatment plant (WWTP) aeration tanks were analyzed for their EDCs contents under optimized conditions. Diltiazem was found in all the samples in the range between 116.4-180.8 ng/g while progesterone and estrone were not detected in any of the samples analyzed with the exception of Tatlar WWTP. The BBP concentration was between beyond detection and 9195.5 ng/g. In addition, Cbz was found in all the samples ranging from 25.6 to 118.8 ng/g.