Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation

ÇALIK, PINAR; Inankur, Bahar; Soyaslan, Elif; Sahin, Merve; Taspinar, Hatice; Acik, Eda; Bayraktar, Eda

doi:10.1002/jctb.2292

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation

ÇALIK P., Inankur B., Soyaslan E. S., Sahin M., Taspinar H., Acik E., ...Daha Fazla

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.85, sa.2, ss.226-233, 2010 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 85 Sayı: 2
Basım Tarihi: 2010
Doi Numarası: 10.1002/jctb.2292
Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.226-233
Anahtar Kelimeler: P. pastoris, human growth hormone, sorbitol, methanol, feeding rate, oxygen transfer, ALKALINE PROTEASE FERMENTATION, CARBON-SOURCES, BACILLUS-LICHENIFORMIS, SECRETION, STRATEGY, CULTURES, ACID
Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) in medium containing sorbitol was investigated at three different specific growth rates (mu), namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 (MS-0.04).