Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed-batch operation


ÇALIK P. , Inankur B., Soyaslan E. S. , Sahin M., Taspinar H., Acik E., ...More

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.85, no.2, pp.226-233, 2010 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 85 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1002/jctb.2292
  • Title of Journal : JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Page Numbers: pp.226-233
  • Keywords: P. pastoris, human growth hormone, sorbitol, methanol, feeding rate, oxygen transfer, ALKALINE PROTEASE FERMENTATION, CARBON-SOURCES, BACILLUS-LICHENIFORMIS, SECRETION, STRATEGY, CULTURES, ACID

Abstract

BACKGROUND: The influence of methanol feed rate on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) in medium containing sorbitol was investigated at three different specific growth rates (mu), namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04 (MS-0.04).