Molecular detection of Bacillus anthracis: evaluation of the efficiency of DNA extraction and a novel dry PCR

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Ortatatli M., Kenar L., Sezigen S., Eyison K., Oktem H. A.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.44, no.2, pp.147-152, 2019 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 44 Issue: 2
• Publication Date: 2019
• Doi Number: 10.1515/tjb-2018-0292
• Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
• Page Numbers: pp.147-152
• Middle East Technical University Affiliated: Yes

Abstract

Background: Due to recent increase in mailings of anthrax spores, the detection of bioweapons has gained a great deal of interest. This study aimed to investigate the yield and purity of DNA obtained from spores and vegetative forms of Bacillus anthracis for detection by conventional (wet) and dry (lyophilized) PCR methods.