Molecular detection of Bacillus anthracis: evaluation of the efficiency of DNA extraction and a novel dry PCR


Ortatatli M., Kenar L., Sezigen S., Eyison K., Oktem H.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.44, sa.2, ss.147-152, 2019 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Konu: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1515/tjb-2018-0292
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Sayfa Sayıları: ss.147-152

Özet

Background: Due to recent increase in mailings of anthrax spores, the detection of bioweapons has gained a great deal of interest. This study aimed to investigate the yield and purity of DNA obtained from spores and vegetative forms of Bacillus anthracis for detection by conventional (wet) and dry (lyophilized) PCR methods.