Immobilization of invertase on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole

Tuncagil S., Kiralp S., Varls S., Toppare L.

REACTIVE & FUNCTIONAL POLYMERS, vol.68, no.3, pp.710-717, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.1016/j.reactfunctpolym.2007.11.010
  • Journal Name: REACTIVE & FUNCTIONAL POLYMERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.710-717
  • Keywords: invertase, enzyme immobilization, electrochemical polymerization, conducting polymers, ACID INVERTASE, BETA-VULGARIS, MATRICES, SUCROSE, MEMBRANE, GLUCOSE, STORAGE, ENZYME
  • Middle East Technical University Affiliated: Yes

Abstract

In this study, immobilization of invertase was achieved on a conducting polymer of 1-(4-nitrophenyl)-2,5-di(2-thienyl)-1H-pyrrole) (SNS (NO2)) via electrochemical polymerization. Kinetic parameters, maximum reaction rate (V-max) and substrate affinity (K-m), optimum temperature and pH, operational and storage stabilities of immobilized enzyme were determined. (c) 2007 Elsevier Ltd. All rights reserved.