A Novel DAD Type and Folic Acid Conjugated Fluorescent Monomer as a Targeting Probe for Imaging of Folate Receptor Overexpressed Cells

Kanik F. E., Ag D., Seleci M., Barlas F. B., Kesik M., Hizalan G., ...More

BIOTECHNOLOGY PROGRESS, vol.30, no.4, pp.952-959, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 4
  • Publication Date: 2014
  • Doi Number: 10.1002/btpr.1928
  • Journal Name: BIOTECHNOLOGY PROGRESS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.952-959
  • Middle East Technical University Affiliated: Yes

Abstract

We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2,3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy. (C) 2014 American Institute of Chemical Engineers