Monitoring Molecular Assembly of Biofilms Using Quartz Crystal Microbalance with Dissipation (QCM-D)

YÜCA YILMAZ E., ŞEKER U. Ö. Ş.

Methods in Molecular Biology, Humana Press Inc., ss.25-33, 2022 identifier identifier

  • Yayın Türü: Kitapta Bölüm / Araştırma Kitabı
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/978-1-0716-2529-3_3
  • Yayınevi: Humana Press Inc.
  • Sayfa Sayıları: ss.25-33
  • Anahtar Kelimeler: Adsorption, Amyloid, Binding, Biofilm, Dissipation, Kinetics, Protein, QCM-D, Quartz sensor, Surface
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

The structure and the functionality of biofilm proteins, the main components of the extracellular matrix, can be tuned by protein engineering. The use of binding kinetics data has been demonstrated in the characterization of recombinantly produced biofilm proteins to control their behavior on certain surfaces or under certain conditions. Quartz crystal microbalance with dissipation monitoring (QCM-D) allows measuring the change in resonance frequency and the energy loss and distribution upon the interaction of molecules with the surface. The characterization of the molecular assembly of curli biofilm proteins on different surfaces using QCM-D is presented here as a detailed protocol. The experimental procedure detailed in this chapter can be applied and modified for other biofilm proteins or subunits to determine their surface adsorption and kinetic binding characteristics.