The effect of crosslinking on thermal inactivation of cellulases


Bilen J., Bakir U.

International Symposium on the Stability and Stabilization of Biocatalysts, Cordoba, Argentina, 19 - 22 April 1998, vol.15, pp.151-156 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume: 15
  • City: Cordoba
  • Country: Argentina
  • Page Numbers: pp.151-156

Abstract

Cellulase complex was crosslinked by using three different crosslinkers, dimethyl suberimidate (DMS), dimethyl adipimidate (DMA) and dimethyl-3-3'-dithiobispropionimidate (DTBP) and their effects on thermal inactivation were investigated. These reagents are all homobifunctional bisimidoesters having different crosslinking distances and crosslink only amino groups to each other. No significant activity loss was observed due to crosslinking with none of the crosslinkers used. In general, DMA and DTBP crosslinking decreased the thermal stability slightly, however with DMS crosslinking, slight increases in thermal stabilities were observed.