Tissue engineered cartilage on collagen and PHBV matrices


Kose G., Korkusuz F. , Ozkul A., Soysal Y., Ozdemir T., Yildiz C., ...More

BIOMATERIALS, vol.26, no.25, pp.5187-5197, 2005 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 25
  • Publication Date: 2005
  • Doi Number: 10.1016/j.biomaterials.2005.01.037
  • Title of Journal : BIOMATERIALS
  • Page Numbers: pp.5187-5197
  • Keywords: cartilage, tissue engineering, collagen, PHBV, biomaterials, ARTICULAR-CARTILAGE, CULTURED CHONDROCYTES, OSTEOCHONDRAL DEFECTS, IN-VITRO, REPAIR, GEL

Abstract

Cartilage engineering is a very novel approach to tissue repair through use of implants. Matrices of collagen containing calcium phosphate (CaP-Gelfix (R)), and matrices of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) were produced to create a cartilage via tissue engineering. The matrices were characterized by scanning electron microscopy (SEM) and electron diffraction spectroscopy (EDS). Porosity and void volume analysis were carried out to characterize the matrices. Chondrocytes were isolated from the proximal humerus of 22 week-old male, adult, local albino rabbits. For cell type characterization, Type II collagen was measured by Western Blot analysis. The foams were seeded with 1 x 10(6) chondrocytes and histological examinations were carried out to assess cell-matrix interaction. Macroscopic examination showed that PHBV (with or without chondrocytes) maintained its integrity for 21 days, while CaP-Gelfix (R) was deformed and degraded within 15 days.