Synthesis and Characterization of Polyhydroxybutyrate Coated Magnetic Nanoparticles: Toxicity Analyses on Different Cell Lines

Yalcin S., Khodadust R., Unsoy G., Garip I. C., Mumcuoglu Z. D., GÜNDÜZ U.

SYNTHESIS AND REACTIVITY IN INORGANIC METAL-ORGANIC AND NANO-METAL CHEMISTRY, vol.45, no.5, pp.700-708, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 5
  • Publication Date: 2015
  • Doi Number: 10.1080/15533174.2013.831448
  • Journal Name: SYNTHESIS AND REACTIVITY IN INORGANIC METAL-ORGANIC AND NANO-METAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.700-708
  • Keywords: polyhydroxybutyrate, magnetic nanoparticle, anticancer agents, cytotoxicity, genotoxicity, IRON-OXIDE, IN-VITRO, POLYHYDROXYALKANOATES, FUNCTIONALIZATION, NANOTECHNOLOGY, DEGRADATION, THERAPY, ASSAY, GENE
  • Middle East Technical University Affiliated: Yes

Abstract

In this study, polyhydroxybutyrate (PHB) coated magnetic nanoparticles were prepared which are targetable to tumor cells in the presence of a magnetic field. The structural properties, functional groups, size distribution, and magnetic properties of the synthesized PHB coated magnetic nanoparticles were characterized by X-ray diffraction, Fourier transform infrared spectrometer, transmission electron microscopy, dynamic light scattering, vibrating sample magnetometry, and thermogravimetric analysis. PHB coated nanoparticles are efficiently internalized by the cancer cells in culture, without cytotoxicity. The results demonstrated that PHB coated iron oxide nanoparticles are suitable for targeted delivery of drugs such as chemotherapeutics, siRNA, miRNA, or antibodies.