INTERESTING FEATURES OF AN ACTIVATED CARBON-SUPPORTED UREASE SYSTEM

KIBARER G.

ARTIFICIAL ORGANS, cilt.18, sa.3, ss.222-226, 1994 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 3
  • Basım Tarihi: 1994
  • Doi Numarası: 10.1111/j.1525-1594.1994.tb02180.x
  • Dergi Adı: ARTIFICIAL ORGANS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.222-226
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

Spherical activated carbon was employed as a new support material for the immobilization of urease. Activity and kinetic behavior of the enzyme were observed. Urease activity was decreased by 10% for the uncoated samples during the washing process whereas a 30% reduction in activity was observed for the coated samples due to the electrical discharge. Activity versus time plots generally appeared to be s-shaped curves at different initial urea levels. At high substrate concentration the kinetics no longer obeyed the Michaelis-Menten relationship due to the inhibition mechanism and diffusion for the support system.