INTERESTING FEATURES OF AN ACTIVATED CARBON-SUPPORTED UREASE SYSTEM

KIBARER G.

ARTIFICIAL ORGANS, vol.18, no.3, pp.222-226, 1994 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 3
  • Publication Date: 1994
  • Doi Number: 10.1111/j.1525-1594.1994.tb02180.x
  • Journal Name: ARTIFICIAL ORGANS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.222-226
  • Middle East Technical University Affiliated: No

Abstract

Spherical activated carbon was employed as a new support material for the immobilization of urease. Activity and kinetic behavior of the enzyme were observed. Urease activity was decreased by 10% for the uncoated samples during the washing process whereas a 30% reduction in activity was observed for the coated samples due to the electrical discharge. Activity versus time plots generally appeared to be s-shaped curves at different initial urea levels. At high substrate concentration the kinetics no longer obeyed the Michaelis-Menten relationship due to the inhibition mechanism and diffusion for the support system.