Demonstration and optimization of sequential microaerobic dark- and photo-fermentation biohydrogen production by immobilized Rhodobacter capsulatus JP91


Sagir E., Yucel M., Hallenbeck P. C.

BIORESOURCE TECHNOLOGY, vol.250, pp.43-52, 2018 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 250
  • Publication Date: 2018
  • Doi Number: 10.1016/j.biortech.2017.11.018
  • Journal Name: BIORESOURCE TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.43-52
  • Keywords: Biohydrogen, Immobilized system, Sequential fermentation, Rhodobacter capsulatus, Response surface methodology, PHOTOFERMENTATIVE HYDROGEN-PRODUCTION, RHODOPSEUDOMONAS-FAECALIS RLD-53, SINGLE-STAGE PHOTOFERMENTATION, RESPONSE-SURFACE METHODOLOGY, CLOSTRIDIUM-BUTYRICUM, PHOTOSYNTHETIC BACTERIUM, GLUCOSE, YIELD, COCULTURE, PHOTOPRODUCTION

Abstract

Hydrogen generation from complex substrates composed of simple sugars has the potential to mitigate future worldwide energy demand. The biohydrogen potential of a sequential microaerobic dark- and photo-fermentative system was investigated using immobilized Rhodobacter capsulatus JP91. Biological hydrogen production from glucose was carried out using a batch process and a bench-scale bioreactor. Response surface methodology with a Box-Behnken design was employed to optimize key parameters such as inoculum concentration, oxygen concentration, and glucose concentration. The maximum hydrogen production (21 +/- 0.25 mmol H-2/L) and yield (7.8 +/- 0.1 mol H-2/mol glucose) were obtained at 6 mM glucose, 4.5% oxygen and 62.5 v/v% inoculum concentration, demonstrating the feasibility of enhanced hydrogen production by immobilized R. capsulatus JP91 in a sequential system. This is the first time that a sequential process using an immobilized system has been described. This system also achieved the highest hydrogen yield obtained by an immobilized system so far.