Studying Tunicata WBR Using Botrylloides anceps

Creative Commons License

Karahan A., Öztürk E., Temiz B., Blanchoud S.

Whole-Body Regeneration Methods and Protocols, Simon Blanchoud,Brigitte Galliot, Editör, Humana Press, Inc. , New York, ss.311-334, 2022

  • Yayın Türü: Kitapta Bölüm / Araştırma Kitabı
  • Basım Tarihi: 2022
  • Yayınevi: Humana Press, Inc.
  • Basıldığı Şehir: New York
  • Sayfa Sayıları: ss.311-334
  • Editörler: Simon Blanchoud,Brigitte Galliot, Editör
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Tunicates are marine filter-feeding invertebrates that can be found worldwide and which are the closest phylogenetic group to the vertebrates (Craniata). Of particular interest, colonial tunicates are the only known chordates that can undergo Whole-Body Regeneration (WBR) via vascular budding. In Botrylloides anceps, a fully functional adult regenerates from a fragment of the vascular system in around 2 weeks after amputation. In this chapter, we present protocols to collect B. anceps colonies, confirm their species, breed them in the lab, monitor WBR and perform histological staining on cryosections.