Enzyme electrodes for determination of total phenolic capacity of red wines

KIRALP S., Cirpan A., TOPPARE L. K.

JOURNAL OF APPLIED POLYMER SCIENCE, cilt.98, sa.1, ss.521-524, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 98 Sayı: 1
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1002/app.22165
  • Dergi Adı: JOURNAL OF APPLIED POLYMER SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.521-524
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

A tyrosinase-modified electrode, based on physical entrapment of enzyme in copolymer matrix of decanedioic acid bis(4-pyrrol-1-yl-phenyl) ester (DAPE) with pyrrole was constructed and used to investigate the total amount of phenolics in red wines. Kinetic parameters, V-max, and K-m, were determined. Also, temperature, pH optimizations, operational stability and shelf life of the enzyme electrode were examined. Surface morphologies of the films were studied by scanning electron microscope. (c) 2005 Wiley Periodicals, Inc.