Surface Occupancy Plays a Major Role in Cholesterol's Condensing Effect


Krause M. R. , Turkyilmaz S. , Regen S. L.

LANGMUIR, cilt.29, sa.33, ss.10303-10306, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Konu: 33
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1021/la402263w
  • Dergi Adı: LANGMUIR
  • Sayfa Sayıları: ss.10303-10306

Özet

The condensing power of cholesterol and 5 alpha-cholestane has been examined in liposomal membranes made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Quantitative nearest-neighbor recognition (NNR) analysis and fluorescence measurements using phase-sensitive probe Laurdan have demonstrated that 5 alpha-cholestane exhibits a substantially weaker condensing effect. This fact, in and of itself, provides compelling evidence that cholesterol's condensing effect is critically dependent on having its steroid nucleus at the membrane surface.