Biohydrogen production by Rhodobacter capsulatus Hup(-) mutant in pilot solar tubular photobioreactor


Boran E., Ozgur E., Yucel M., GÜNDÜZ U. , EROĞLU İ.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.37, no.21, pp.16437-16445, 2012 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 21
  • Publication Date: 2012
  • Doi Number: 10.1016/j.ijhydene.2012.02.171
  • Title of Journal : INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Page Numbers: pp.16437-16445
  • Keywords: Biohydrogen, Pilot, Tubular photobioreactor, Rhodobacter capsulatus, Hup(-) mutant, Photofermentation, BIOLOGICAL HYDROGEN-PRODUCTION, POLY-BETA-HYDROXYBUTYRATE, SPHAEROIDES, BIOMASS, OUTDOOR, H-2

Abstract

In this study, a pilot solar tubular photobioreactor was successfully implemented for fed batch operation in outdoor conditions for photofermentative hydrogen production with Rhodobacter capsulatus (Hup(-)) mutant. The bacteria had a rapid growth with a specific growth rate of 0.052 h(-1) in the batch exponential phase and cell dry weight remained in the range of 1-1.5 g/L throughout the fed batch operation. The feeding strategy was to keep acetic acid concentration in the photobioreactor at the range of 20 mM by adjusting feed acetate concentration. The maximum molar productivity obtained was 0.40 mol H-2/(m(3) h) and the yield obtained was 0.35 mol H-2 per mole of acetic acid fed. Evolved gas contained 95-99% hydrogen and the rest was carbon dioxide by volume. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.