Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars

Kayihan C., EYİDOĞAN F., Afsar N., ÖKTEM H. A. , YÜCEL M.

BIOLOGIA PLANTARUM, vol.56, no.4, pp.693-698, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.1007/s10535-012-0143-x
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.693-698
  • Keywords: antioxidant enzymes, Hordeum vulgare, low temperature, rewarming, OXIDATIVE-STRESS, ARABIDOPSIS, TOLERANCE, SEEDLINGS, WHEAT, RESPONSES, PLANTS, ACID
  • Middle East Technical University Affiliated: Yes


The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (-3 A degrees C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (-7 A degrees C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity.