Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars


Kayihan C., EYİDOĞAN F., Afsar N., ÖKTEM H. A. , YÜCEL M.

BIOLOGIA PLANTARUM, cilt.56, ss.693-698, 2012 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 56 Konu: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s10535-012-0143-x
  • Dergi Adı: BIOLOGIA PLANTARUM
  • Sayfa Sayıları: ss.693-698

Özet

The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (-3 A degrees C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (-7 A degrees C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity.