In this study, amperometric biosensing performance of a novel platform utilizing a conducting polymer (CP), multi-walled carbon nanotubes (MWCNTs) and poly(amidoamine) (PAMAM) dendrimer was constructed. In order to fabricate the proposed biosensor, a graphite electrode was modified with poly[9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl] end capped with N,N-bis(4-methylphenyl)-4-aniline (PFLA), MWCNTs and PAMAM, respectively. Then, GOx was immobilized onto the modified surface with the help of glutaraldehyde (GA) as the crosslinking reagent. Then, analytical and kinetic parameters of the constructed biosensor were investigated and the biosensor showed a linear response for glucose between 0.05 and 0.75 mM with a detection limit of 0.014 mM. K-M(app) and sensitivity values were calculated as 0.66 mM and 55.41 mu A mM(-1) cm(-2), respectively. To investigate the surface modifications, scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques were used. Finally, fabricated biosensor was tested on beverages for glucose detection successfully.