Investigation of SREBP-1 and C/EBPβ expression during global ischemia/reperfusion-induced oxidative stress in rat brain cortex and cerebellum
Turkish Journal of Biochemistry, 2026 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1515/tjb-2025-0362
- Dergi Adı: Turkish Journal of Biochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Applied Science & Technology Source, EMBASE, Food Science & Technology Abstracts, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
- Anahtar Kelimeler: carotid artery occlusion, GST, SOD, TBARS, Western blot
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Objectives: Ischemic brain injury causes neurodegeneration. This study investigated the mechanism of neurodegeneration by examining the expression of transcription factors, sterol regulatory element-binding protein-1 (SREBP-1) and CCAAT enhancer-binding protein β (C/EBPβ), in a time course. Besides oxidative stress markers such as thiobarbituric acid-reactive substances (TBARS), total thiol molecule (TTM) levels, and superoxide dismutase (SOD), glutathione-S-transferase (GST) activities were also detected. Methods: In adult male rats, carotid artery occlusion and hypotension were produced for 10 min. Control groups were sham-operated. Animals were sacrificed after 24 h, 1, 2, and 4 weeks of reperfusion periods. The expression of SREBP-1 and C/EBPβ in the rat brain cortex and cerebellum was examined by Western blotting. Results: C/EBPβ expression significantly increased in both cytosolic (1.19-, 1.58-fold) and nuclear (1.73-, 1.81-fold) extracts of the brain cortex after 24 h and 1 week of reperfusion. In the cerebellum, C/EBPβ expression significantly increased in 1 week, cytosolic (1.63-fold), and nuclear (1.35-fold) extracts. SREBP-1 expression significantly increased in both cytosolic (2.07-fold) and nuclear (1.41-fold) extracts of the brain cortex after 1 week of reperfusion. SREBP-1 expression significantly increased in cytosolic (2.15-fold) and nuclear (1.79-fold) extracts of cerebellum after 1 week of reperfusion. In addition, TBARS levels and SOD activities significantly increased by 43.16 % and 47.30 %, respectively, after 24 h of reperfusion. Conclusions: Our findings indicate that an increase in oxidative stress parameters arose at the earlier phase of ischemic neurodegenerative processes, while SREBP-1 expression increased in the mid-phase. C/EBPβ expressions were increased at early to mid-phases of reperfusion injury.