Recombinant protein production in Pichia pastoris: From transcriptionally redesigned strains to bioprocess optimization and metabolic modelling


Ergün B. G. , Berrios J., Binay B., Fickers P.

FEMS Yeast Research, vol.21, no.7, 2021 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Review
  • Volume: 21 Issue: 7
  • Publication Date: 2021
  • Doi Number: 10.1093/femsyr/foab057
  • Journal Name: FEMS Yeast Research
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE
  • Keywords: Pichia pastoris (Kornagataella phaffii), heterologous protein production, transcriptional circuitry design, promoter engineering, non-conventional carbon source-based bioprocess design and optimization, metabolic modelling, SYNTHETIC CORE PROMOTERS, RHIZOPUS-ORYZAE LIPASE, FLUX BALANCE ANALYSIS, FED-BATCH, FEEDING STRATEGY, KOMAGATAELLA-PHAFFII, MACROKINETIC MODEL, LOW-TEMPERATURE, CARBON-SOURCES, MIXED-FEEDS

Abstract

© 2021 The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.Pichia pastoris is one of the most widely used host for the production of recombinant proteins. Expression systems that rely mostly on promoters from genes encoding alcohol oxidase 1 or glyceraldehyde-3-phosphate dehydrogenase have been developed together with related bioreactor operation strategies based on carbon sources such as methanol, glycerol, or glucose. Although, these processes are relatively efficient and easy to use, there have been notable improvements over the last twenty years to better control gene expression from these promoters and their engineered variants. Methanol-free and more efficient protein production platforms have been developed by engineering promoters and transcription factors. The production window of P. pastoris has been also extended by using alternative feedstocks including ethanol, lactic acid, mannitol, sorbitol, sucrose, xylose, gluconate, formate or rhamnose. Herein, the specific aspects that are emerging as key parameters for recombinant protein synthesis are discussed. For this purpose, a holistic approach has been considered to scrutinize protein production processes from strain design to bioprocess optimization, particularly focusing on promoter engineering, transcriptional circuitry redesign. This review also considers the optimization of bioprocess based on alternative carbon sources and derived co-feeding strategies. Optimization strategies for recombinant protein synthesis through metabolic modelling are also discussed.