Effect of progesterone on DPPC membrane: Evidence for lateral phase separation and inverse action in lipid dynamics

Korkmaz F., Severcan F.

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, cilt.440, sa.2, ss.141-147, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 440 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.abb.2005.06.013
  • Dergi Adı: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.141-147
  • Anahtar Kelimeler: DPPC liposorne, progesterone, FTIR, DSC, turbidity, DIFFERENTIAL SCANNING CALORIMETRY, TRANSFORM INFRARED-SPECTROSCOPY, MODEL MEMBRANES, ALPHA-TOCOPHEROL, FLUIDITY, PHOSPHATIDYLCHOLINE, DIPALMITOYLPHOSPHATIDYLCHOLINE, VITAMIN-D-2, TRANSITION, ESTRADIOL
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

Interactions of progesterone with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) triultilamellar liposomes were investigated as a function of temperature and progesterone concentration by using three non-invasive techniques namely Fourier transform infrared spectroscopy, turbidity at 440 nm, and differential scanning calorimetry. The results reveal that progesterone changes the physical properties of DPPC bilayers by decreasing the main phase-transition temperature, abolishing the pre-transition, broadening the phase-transition profile, disordering the system both in gel and liquid crystalline phase, increasing the dynamics at low concentrations whereas stabilizing the membrane at high concentrations, and inducing phase separation. Progesterone does not change the hydration of the C=O groups, while it strengthens the hydrogen bonding between the PO (2) over bar groups of lipids and the water molecules around. (c) 2005 Elsevier Inc. All rights reserved.