The Synthesis of Complex Polymer Electrolytes Based on Alginic Acid and Poly(1-vinylimidazole) and Application in Tyrosinase Immobilization


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Kartal M., Kayahan S. K. , Bozkurt A., Toppare L.

POLYMER JOURNAL, cilt.41, ss.46-50, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 41 Konu: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1295/polymj.pj2008130
  • Dergi Adı: POLYMER JOURNAL
  • Sayfa Sayıları: ss.46-50

Özet

In this study, proton conducting polymer electrolyte networks consisting of alginic acid (AA) and poly(1-vinylimidazole) (PVI) were prepared. The polymer networks were obtained by mixing AA and PVI with several stoichiometric ratios, x (with respect to monomers). Polymer networks were characterized by FT-IR spectroscopy and their compositions were investigated by elemental analysis (EA). Enzyme entrapped polymer networks (EEPN) were produced by immobilization of tyrosinase in the AA/PVI matrix during complexation. The maximum reaction rate (V-max) and Michaelis-Menten constant (K-m) were investigated for the immobilized tyrosinase. Also, the temperature and pH optimizations, operational stability and shelf life of enzyme immobilized in the polymer network were examined.