The Synthesis of Complex Polymer Electrolytes Based on Alginic Acid and Poly(1-vinylimidazole) and Application in Tyrosinase Immobilization
POLYMER JOURNAL, vol.41, no.1, pp.46-50, 2009 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 41 Issue: 1
- Publication Date: 2009
- Doi Number: 10.1295/polymj.pj2008130
- Journal Name: POLYMER JOURNAL
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.46-50
- Keywords: Alginic Acid, Poly(1-vinylimidazole), Immobilized Enzymes, Biosensors, Proton Conductivity, Kinetic Parameters, PROTON-CONDUCTING POLYMERS, SPECTROPHOTOMETRIC METHOD, VINYLPHOSPHONIC ACID, POLYPHENOL OXIDASE, PHENOLIC-COMPOUNDS, COMPOSITE-MATERIAL, PHOSPHORIC-ACID, IMIDAZOLE, COPOLYMERS, ELECTRODES
- Open Archive Collection: AVESIS Open Access Collection
- Middle East Technical University Affiliated: Yes
Abstract
In this study, proton conducting polymer electrolyte networks consisting of alginic acid (AA) and poly(1-vinylimidazole) (PVI) were prepared. The polymer networks were obtained by mixing AA and PVI with several stoichiometric ratios, x (with respect to monomers). Polymer networks were characterized by FT-IR spectroscopy and their compositions were investigated by elemental analysis (EA). Enzyme entrapped polymer networks (EEPN) were produced by immobilization of tyrosinase in the AA/PVI matrix during complexation. The maximum reaction rate (V-max) and Michaelis-Menten constant (K-m) were investigated for the immobilized tyrosinase. Also, the temperature and pH optimizations, operational stability and shelf life of enzyme immobilized in the polymer network were examined.