Whole-genome sequencing of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae from retail raw milk

ASLANTAŞ Ö., YILMAZ E. Ş., Buyukaltay K., Kocabag H. D.

INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, vol.75, no.1, pp.77-82, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 75 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.1111/1471-0307.12828
  • Journal Name: INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
  • Page Numbers: pp.77-82
  • Keywords: Enterobacteriaceae, ESBL, pAmpC, Raw milk, Whole-genome sequencing, BULK TANK MILK
  • Middle East Technical University Affiliated: Yes

Abstract

The contaminated products of food-producing animals have been proposed to lead the transmission of resistant bacteria to consumers. Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, which were isolated from raw milk samples, were characterised using whole-genome sequencing in this study. Three confirmed ESBL and one plasmid-mediated AmpC beta-lactamase (pAmpC)-producing isolate were obtained. All of the ESBL-producing isolates showed a multidrug-resistant (MDR) phenotype, but the pAmpC-producing isolate was only resistant to beta-lactams. This study showed that raw milk can contain MDR bacteria that have several resistance determinants and highlighted the necessity of efficiently and routinely screening the products of food-producing animals.