This study aims to develop valid and reliable scale in order to measure preservice teachers' beliefs towards using origami in mathematics education. Origami in Mathematics Education Belief Scale which consists of 27 items with 6 point Likert type was administered to 143 preservice elementary mathematics teachers and obtained data was analyzed with exploratory factor analysis. According to analysis results, one item was deleted and some items were revised. After these revisions, the scale consists of 26 items under two dimensions. First dimension is benefits of origami in mathematics education consisting of 19 items and second dimension is limitations of using origami in mathematics education consisting of 7 items. This last version of the scale was administered to 299 preservice elementary mathematics teachers and obtained data was analyzed via confirmatory factor analysis. According to confirmatory factor analysis, RMSEA was calculated as 0.091, CFI and GFI values were calculated as 0.90.