Two new species, Harpactea arnedoi sp. n. and H. kencei sp. n., are described on the basis of both sexes from the eastern Mediterranean and Aegean regions of Turkey. H. kencei sp. n. can be easily distinguished from all other Turkish and European representatives of the genus by the structure of the flattened, massive embolus on the male copulatory organ. Although resembling Stalagtia in palpal morphology, we describe one of the new species as Harpactea arnedoi sp. n. For both Turkish species, detailed morphological descriptions and diagnoses are presented together with figures of the copulatory organs.