Web accessibility means that disabled people can effectively perceive, understand, navigate, and interact with the web. Web accessibility evaluation methods are needed to validate the accessibility of web pages. However, the role of subjectivity and of expertise in such methods is unknown and has not previously been studied. This article investigates the effect of expertise in web accessibility evaluation methods by conducting a Barrier Walkthrough (BW) study with 19 expert and 57 nonexpert judges. The BW method is an evaluation method that can be used to manually assess the accessibility of web pages for different user groups such as motor impaired, low vision, blind, and mobile users.