Immobilization of tyrosinase in polysiloxane/polypyrrole copolymer matrices

Arslan A., Kiralp S., Toppare L., Yagci Y.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.35, pp.163-167, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 35
  • Publication Date: 2005
  • Doi Number: 10.1016/j.ijbiomac.2005.01.006
  • Journal Name: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.163-167
  • Keywords: immobilization, tyrosinase, conducting copolymers, polysiloxane, electrochemical polymerization, enzyme electrode, POLYPHENOL OXIDASE, CONDUCTING COPOLYMERS, INVERTASE
  • Middle East Technical University Affiliated: Yes

Abstract

Immobilization of tyrosinase in conducting copolymer matrices of pyrrole functionalized polydimethylsiloxane/polypyrrole (PDMS/PPy) was achieved by electrochemical polymerization. The polysiloxane/polypyrrole/tyrosinase electrode was constructed by the entrapment of enzyme in conducting matrices during electrochemical copolymerization. Maximum reaction rate (V-max) and Michaelis-Menten constant were investigated for immobilized enzyme. Enzyme electrodes were prepared in two different electrolyte/sol vent systems. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate on the enzyme activity and film morphology were determined. Temperature and pH optimization, operational stability and shelf-life of enzyme electrodes were also examined. Phenolic contents of green and black tea were determined by using enzyme electrodes. (c) 2005 Elsevier B.V. All rights reserved.