A novel aerobic sludge digestion process to stabilize and decrease the amount of excess sludge produced during biological treatment and removal of EDCs sorbed onto sludge during this process is discussed here. Waste activated sludge samples from two different wastewater treatment plants were ozonated for different periods in Erlenmeyer flasks once a day on each of four consecutive days. Flasks were continuously aerated between ozone applications by shaking on an orbital shaker. The residual EDC concentrations in sludge samples were analyzed at the end of digestion periods. An MLVSS reduction of up to 95% was achieved with an ozone dose of only 1.05 g O-3/kg MLSS in this process on the fourth day. During this process destruction of some selected endocrine disrupting compounds, namely diltiazem, carbamazepine, butyl benzyl phthalate, acetaminophen and two natural hormones, estrone and progesterone, which tend to accumulates in sludge, are studied. Over 99% removal of these contaminants were achieved at the end of the fourth day. The analyses were conducted by using LC (ESI) MS/MS after solid phase extraction (SPE). By this process it became possible to save on contact time as well as achieving a bio-solids digestion far exceeding the standard aerobic process and concomitant removal of micropollutants at the expense of minimum ozone dose. The developed process is deemed superior over side-stream ozonation of activated sludge, in that it does not cause reduction in active biomass in the aeration tank. (C) 2012 Elsevier B.V. All rights reserved.