Akademik Veri Yönetim Sistemi
Viruses of Microbes 2023, Tbilisi, Gürcistan, 3 - 07 Temmuz 2023, ss.118
Phage endolysins against Salmonella
Segâh Yetiskin1, Aysenur Yucefaydali1, Yeşim Soyer1,2*
1 Department of Food Engineering, Middle East Technical University. Ankara, Turkey.
2 Department of Biotechnology, Middle East Technical University. Ankara, Turkey
Salmonella is one of the major bacteria causing foodborne diseases in the world. Salmonella is gram negative, non-spore forming and rod shape bacteria, and those with enteric fever and non-typhoidal salmonellosis cause gastroenteritis problems. Bacteriophages are the viruses that infect bacterial cells. Endolysins, bacteriophage lytic enzymes, are enzymes produced during growth of bacteriophage in the cell. Endolysins cause cell death by disturbing cell wall and cell membrane of the bacteria and to release new forming viruses. Lysins aim important and conserved regions of the cell which allow to release new viruses. Because of these features, resistance ability of bacteria to endolysins is less than to antibiotics. Since eukaryotes don’t have peptidoglycan, endolysins doesn’t affect human and animals. In this study, bacteriophage, named P1-137, was isolated and characterized and its endolysin was expressed. The genome of the phage was analyzed and genes producing endolysins were selected. Endolysin gene was transferred to E. coli BL21 strain using vector and production was achieved. After production, purification was done and the effects of these proteins on bacteria were investigated.