A novel procedure for the preparation of organosoluble polyo-aminophenol) is described. The method pertains to the reduction of CuBr2 with o-amino phenol OAP) at room temperature. The air oxidation of the CuI) species to Cu(II) facilitated the formation of polyo-aminophenol) POAP) in repeated cycles. The obtained polymer, soluble in common organic solvents such as dimethylformamide, acetone, and chloroform, was characterized by spectral analyses. It was found that both quinoid and benzoid structures were present in the polymer chain. Polyo-aminophenol) architecture was then applied as an immobilization matrix to obtain a long-lived and fast response bio-sensing system. Glucose oxidase GOx) was utilized as a model enzyme for the detection of glucose in beverages. After the optimization studies, the surface characterization of POAP/GOx was performed by scanning electron microscopy SEM) measurements. Under the optimized conditions, the biosensor exhibited a rapid response 2 s), a low detection limit 0.02 mM), and high sensitivity 79.80 mu A mM(-1) cm(-2)). The K-M(app) value was determined as 0.16 mM. Furthermore, there was no considerable activity loss for 15 days for the POAP/GOx biofilm indicating excellent recovery as tested with real samples.