Diversity of the Aromatic-Ring-Hydroxylating Dioxygenases in the Monoaromatic Hydrocarbon Degraders Held by a Common Ancestor

Yavas A., İçgen B.

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, cilt.101, ss.410-416, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 101
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s00128-018-2350-4
  • Dergi Adı: BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.410-416
  • Anahtar Kelimeler: ARHD, Aromatic ring hydroxylating dioxygenases, TodC1, Hydrocarbon degraders, PSEUDOMONAS-PUTIDA F1, ENVIRONMENTAL-SAMPLES, XENOBIOTIC COMPOUNDS, TOLUENE DEGRADATION, ESCHERICHIA-COLI, ACTIVATED-SLUDGE, OXYGENASE, GENES, SPHINGOMONAS, STRAIN
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Aromatic ring hydroxylating dioxygenases (ARHDs), harboured by a variety of bacteria, catalyze the initial reaction in the degradation of a wide range of toxic environmental contaminants like aromatic and polycyclic aromatic hydrocarbons (PAHs). Regardless of the source, bacteria harbouring RHDs play major role in the removal of these toxic contaminants. The diversity of ARHDs in contaminated sites is supposed to be huge. However, most of the ARHD diversity studies are based on the PAH degraders and the ARHD diversity in the monoaromatic hydrocarbon degraders has not fully explored yet. In this study, therefore, the ARHD gene from nine different genara of the monoaromatic hydrocarbon degraders including Raoultella, Stenotrophomons, Staphylococcus, Acinetobacter, Pseudomonas, Serratia, Comamonas, Pantoea, and Micrococcus was analysed through polymerase chain reactions and sequencing. The sequence alignments of the ARHD amplicons with 81%-99% homologies were found to be highly related and held by divergent evolution from a common ancestor.