Influence of Composition of Zeolite/Enzyme Nanobiocomposites on Analytical Characteristics of Urea Biosensor Based on Ion-Selective Field-Effect Transistors


Soldatkin O. O. , Soy E., Errachid A., Jaffrezic-Renault N., AKATA KURÇ B., Soldatkin A. P. , ...More

SENSOR LETTERS, vol.9, no.6, pp.2320-2326, 2011 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 6
  • Publication Date: 2011
  • Doi Number: 10.1166/sl.2011.1809
  • Journal Name: SENSOR LETTERS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.2320-2326
  • Keywords: Zeolite, Nanobiocomposite, Urease, FET Biosensor, Urea, METAL-IONS, ENZYME, MULTIBIOSENSOR

Abstract

Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It ensures as wide linear range of measurement without remarkable loss in biosensor sensitivity to urea. The BEA zeolite-based biosensors were shown to have better working parameters in comparison with those based on zeolites A (LTA). A decrease in biosensor sensitivity to heavy metal ions was demonstrated for all zeolites used, which testifies to probable increase in stability of urea measurement in real environmental samples.