Influence of Composition of Zeolite/Enzyme Nanobiocomposites on Analytical Characteristics of Urea Biosensor Based on Ion-Selective Field-Effect Transistors

Soldatkin O. O., Soy E., Errachid A., Jaffrezic-Renault N., AKATA KURÇ B., Soldatkin A. P., ...Daha Fazla

SENSOR LETTERS, cilt.9, sa.6, ss.2320-2326, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 6
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1166/sl.2011.1809
  • Dergi Adı: SENSOR LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2320-2326
  • Anahtar Kelimeler: Zeolite, Nanobiocomposite, Urease, FET Biosensor, Urea, METAL-IONS, ENZYME, MULTIBIOSENSOR
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Zeolite/enzyme nanobiocomposites of different compositions were tested in this work for the improvement of biosensor analytical characteristics. The bioselective element based on urease immobilized by cross-linking with glutaraldehyde was used as a model. The working characteristics of biosensors based on various zeolite/enzyme nanocomposites were examined and compared with those of urease-based biosensors. An optimal concentration of zeolytes beta (BEA) in bioselective elements is determined to be 1.5%. It ensures as wide linear range of measurement without remarkable loss in biosensor sensitivity to urea. The BEA zeolite-based biosensors were shown to have better working parameters in comparison with those based on zeolites A (LTA). A decrease in biosensor sensitivity to heavy metal ions was demonstrated for all zeolites used, which testifies to probable increase in stability of urea measurement in real environmental samples.