Small molecule detection by lateral flow strips via aptamer-gated silica nanoprobes

Ozalp V. C., Cam D., Hernandez F. J., Hernandez L. I., Schafer T., ÖKTEM H. A.

ANALYST, vol.141, no.8, pp.2595-2599, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 141 Issue: 8
  • Publication Date: 2016
  • Doi Number: 10.1039/c6an00273k
  • Journal Name: ANALYST
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2595-2599
  • Middle East Technical University Affiliated: Yes


A fast, sensitive and ratiometric biosensor strategy for small molecule detection was developed through nanopore actuation. The new platform engineers together, a highly selective molecular recognition element, aptamers, and a novel signal amplification mechanism, gated nanopores. As a proof of concept, aptamer gated silica nanoparticles have been successfully used as a sensing platform for the detection of ATP concentrations at a wide linear range from 100 mu M up to 2 mM.