Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices
EUROPEAN POLYMER JOURNAL, cilt.39, sa.12, ss.2375-2381, 2003 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 39 Sayı: 12
- Basım Tarihi: 2003
- Doi Numarası: 10.1016/s0014-3057(03)00184-8
- Dergi Adı: EUROPEAN POLYMER JOURNAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2375-2381
- Orta Doğu Teknik Üniversitesi Adresli: Evet
Özet
Immobilization of invertase and glucose oxidase in conducting polypyrrole and copolymers of poly 2-methylbutyl-2-(3-thienyl) acetate with pyrrole were achieved via electrochemical method. Sodium dodecyl sulphate was found to be the most suitable supporting electrolyte. Maximum reaction rate, Michaelis-Menten constant and optimum temperatures were determined for native and immobilized enzymes. Storage and operational stabilities of enzyme electrodes were also investigated. (C) 2003 Elsevier Ltd. All rights reserved.