Akademik Veri Yönetim Sistemi
Journal of Orthopaedic Surgery and Research, cilt.20, sa.1, 2025 (SCI-Expanded, Scopus)
Purpose: The ideal treatment of osteochondral defects is controversial. Regenerative treatment methods aim to produce ideal scaffolds using different biomaterials. Decellularized extracellular matrix (ECM) containing scaffolds are good options recently. Promising results have been reported with decellularized human omentum xenografts in animal models for tissue regeneration. Mesenchymal stem cells (MSCs) and growth factors also form other parts of the tissue engineering triad for cartilage regeneration The objective of this study is to evaluate the regenerative effects of the decellularised omentum-based scaffold, MSCs and platelet-rich plasma (PRP) in the rat knee articular cartilage model. To our knowledge, this study is the first to evaluate the combined effect of a decellularized human omentum composite scaffold, mesenchymal stem cells, and PRP on osteochondral defect repair. Methods: Osteochondral defects were bilaterally created in the femoral trochlea of 42 rats. The animals were randomly divided into five groups: negative control (C), scaffold only (O), scaffold combined with mesenchymal stem cells (OM), scaffold combined with PRP (OP) and scaffold combined with both MSCs and PRP (OMP). At 12 weeks postoperatively, cartilage repair was assessed by macroscopic and histomorphometric evaluation. Results: The O group (p = 0.022) and the OMP group (p = 0.001) showed higher scores than the C group macroscopically. No statistically significant differences were observed between the other group comparisons (p > 0.05). According to the total O’Driscoll evaluation scores, none of the experimental groups showed a significant improvement compared to the C group. However, the OMP group had significantly higher total scores than the group O (p = 0.002). According to the ICRS scoring system, the OMP application showed significantly better surface continuity (p = 0.0314), healthier calcified cartilage formation (p = 0.0327), and higher cell viability (p = 0.0029) compared to group O. The OM group demonstrated improved calcified cartilage structure compared to the O group (p = 0.0429). Conclusions: The omentum-based scaffold, along with MSCs and PRP orthobiologic interventions applied in a single-dose, 12-week protocol, demonstrated limited efficacy in osteochondral defect regeneration. Level of evidence: Level NA-Animal studies.