Melatonin induces opposite effects on order and dynamics of anionic DPPG model membranes

Sahin I., Severcan F., Kazanci N.

JOURNAL OF MOLECULAR STRUCTURE, vol.834, pp.195-201, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 834
  • Publication Date: 2007
  • Doi Number: 10.1016/j.molstruc.2006.12.002
  • Journal Name: JOURNAL OF MOLECULAR STRUCTURE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.195-201
  • Keywords: melatonin, dipalmitoyl phosphatidylglycerol, membrane, Fourier transform infrared, differential scanning calorimetry, lipid order, membrane fluidity, hydrogen bonding, DIPALMITOYL PHOSPHATIDYLCHOLINE, FLUORESCENCE ANISOTROPY, LIPOSOMAL MEMBRANE, PHASE-TRANSITIONS, LIPID-BILAYERS, PLUS MELATONIN, IONIC MICELLES, BINDING, VESICLES, DRUGS
  • Middle East Technical University Affiliated: No

Abstract

The temperature and concentration induced effects of melatonin on anionic dipalmitoyl phosphatidylglycerol (DPPG) multilamellar liposomes (MLVs) were investigated by using Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). The results show that melatonin does not perturb the phase transition profile, while a decrease in the main transition temperature (T-m) is noticed at high melatonin concentrations (15, 24 and 30 mol Low concentrations of melatonin (3, 6 and 9 mol %) decrease the frequency of the CH2 stretching mode, implying an ordering effect, whilst high concentrations of melatonin disorders system both in the gel and liquid crystalline phases. Furthermore, at low and high concentrations, melatonin also causes opposite effect on membrane dynamics. The bandwidth of the CH2 stretching modes decreases at low concentrations, implying a decrease in the dynamics, while increasing it at high concentrations. Furthermore, it causes significant decrease in the frequency of the C=O stretching and PO2- antisymmetric double bond stretching bands of DPPG for all concentrations both in the gel and liquid crystalline phases, which indicates strong hydrogen bonding around these functional groups. (C) 2006 Elsevier B.V. All rights reserved.