A smartphone based surface plasmon resonance imaging (SPRi) platform for on-site biodetection


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Guner H. , Ozgur E., Kokturk G., Celik M. , Esen E., Topal A. E. , ...Daha Fazla

SENSORS AND ACTUATORS B-CHEMICAL, cilt.239, ss.571-577, 2017 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 239
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.snb.2016.08.061
  • Dergi Adı: SENSORS AND ACTUATORS B-CHEMICAL
  • Sayfa Sayıları: ss.571-577

Özet

We demonstrate a surface plasmon resonance imaging platform integrated with a smartphone to be used in the field with high-throughput biodetection. Inexpensive and disposable SPR substrates are produced by metal coating of commercial Blu-ray discs. A compact imaging apparatus is fabricated using a 3D printer which allows taking SPR measurements from more than 20.000 individual pixels. Real-time bulk refractive index change measurements yield noise equivalent refractive index changes as low as 4.12 x 10(-5) RIU which is comparable with the detection performance of commercial instruments. As a demonstration of a biological assay, we have shown capture of mouse IgG antibodies by immobilized layer of rabbit anti-mouse (RAM) IgG antibody with nanomolar level limit of detection. Our approach in miniaturization of SPR biosensing in a cost-effective manner could enable realization of portable SPR measurement systems and kits for point-of-care applications. (C) 2016 Published by Elsevier B.V.