Application of Different Zeolites for Improvement of the Characteristics of a pH-FET Biosensor Based on Immobilized Urease

Soldatkin O. O., Kucherenko I. S., Shelyakina M. K., Soy E., Kirdeciler K., ÖZTÜRK S., ...More

ELECTROANALYSIS, vol.25, no.2, pp.468-474, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 2
  • Publication Date: 2013
  • Doi Number: 10.1002/elan.201200475
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.468-474
  • Keywords: Biosensors, pH-FET, Urease, Zeolites, MULTIBIOSENSOR, MONOLAYERS
  • Middle East Technical University Affiliated: Yes


Different modifications of the zeolites Na+-Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co-immobilized with bovine serum albumin on the surface of a pH-FET. It was shown that the biosensors modified with the zeolites H+-Beta30 and H+-Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H+-Beta30 and H+-Beta50 in the bioselective membrane was 15%. Different variants of co-immobilization of urease and zeolite H+-Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H+-Beta30 for urease immobilization in the bioselective membrane.