In this study mutagenic effects of some chemicals used in Veterinary Medicine and agricultural fields was investigated by Somatic Mutation and Recombination Test (SMART). SMART test was to observe the chemical effects on wing phenotype of the trans- heterozygote flies carrying marker gene. Lethal doses of the chemicals used were determined. A positive correlation was observed between total mutation and the number of wings having mutations. In addition, the observed mutations were classified according to the size and the type of the mutations per wing. Chemicals used were ranked as Dichlorvos, Methyl parathion, Azamethyphos according to their genotoxic effects. The present study pointed out that the correct administration of pesdicides has significant effect on human and enviromental health which are under the threat of hazardous pesticid residues.