Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F

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Osmanagaoglu O., Beyatli Y., Gunduz U.

JOURNAL OF BASIC MICROBIOLOGY, vol.40, no.1, pp.41-49, 2000 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 40 Issue: 1
• Publication Date: 2000
• Doi Number: 10.1002/(sici)1521-4028(200002)40:1<41::aid-jobm41>3.0.co;2-#
• Journal Name: JOURNAL OF BASIC MICROBIOLOGY
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.41-49
• Middle East Technical University Affiliated: Yes

Abstract

Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.