Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F


Osmanagaoglu O., Beyatli Y., Gunduz U.

JOURNAL OF BASIC MICROBIOLOGY, cilt.40, sa.1, ss.41-49, 2000 (SCI İndekslerine Giren Dergi) identifier identifier

Özet

Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.