EFFICIENCY OF GENE-TRANSFER BY CONJUGATION AND ELECTROPORATION IN LACTOCOCCI AND PEDIOCOCCI

ALTAY G., BOZOGLU F., RAY B.

FOOD MICROBIOLOGY, cilt.11, sa.4, ss.265-270, 1994 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 4
  • Basım Tarihi: 1994
  • Doi Numarası: 10.1006/fmic.1994.1029
  • Dergi Adı: FOOD MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.265-270
  • Orta Doğu Teknik Üniversitesi Adresli: Hayır

Özet

Sucrose-fermenting ability and nisin-producing traits were transferred to Lactococcus lactis strains by direct plate conjugation. (DPC), solid surface conjugation (SSC) and membrane filter conjugation. (MFC) with frequencies ranging from 2.6 x 10(-6) to 8.9 x 10(-10) transconjugant/donor colony forming unit (cfu). Plasmid pGK12 was transferred into plasmid-free Pediococcus acidilactici F3 by electroporation with a transfer frequency of 49 transformants/mu g DNA used.