EFFICIENCY OF GENE-TRANSFER BY CONJUGATION AND ELECTROPORATION IN LACTOCOCCI AND PEDIOCOCCI


ALTAY G., BOZOGLU F., RAY B.

FOOD MICROBIOLOGY, vol.11, no.4, pp.265-270, 1994 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 4
  • Publication Date: 1994
  • Doi Number: 10.1006/fmic.1994.1029
  • Journal Name: FOOD MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.265-270

Abstract

Sucrose-fermenting ability and nisin-producing traits were transferred to Lactococcus lactis strains by direct plate conjugation. (DPC), solid surface conjugation (SSC) and membrane filter conjugation. (MFC) with frequencies ranging from 2.6 x 10(-6) to 8.9 x 10(-10) transconjugant/donor colony forming unit (cfu). Plasmid pGK12 was transferred into plasmid-free Pediococcus acidilactici F3 by electroporation with a transfer frequency of 49 transformants/mu g DNA used.