Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts


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Polanska U. M. , ACAR A. , Orimo A.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no.56, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: Issue: 56
  • Publication Date: 2011
  • Doi Number: 10.3791/3201
  • Title of Journal : JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

Abstract

Carcinomas are complex tissues comprised of neoplastic cells and a non-cancerous compartment referred to as the 'stroma'. The stroma consists of extracellular matrix (ECM) and a variety of mesenchymal cells, including fibroblasts, myofibroblasts, endothelial cells, pericytes and leukocytes(1-3).