Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts


Creative Commons License

Polanska U. M. , ACAR A., Orimo A.

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no.56, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: Issue: 56
  • Publication Date: 2011
  • Doi Number: 10.3791/3201
  • Journal Name: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Middle East Technical University Affiliated: Yes

Abstract

Carcinomas are complex tissues comprised of neoplastic cells and a non-cancerous compartment referred to as the 'stroma'. The stroma consists of extracellular matrix (ECM) and a variety of mesenchymal cells, including fibroblasts, myofibroblasts, endothelial cells, pericytes and leukocytes(1-3).