Domoic acid production by <i>Pseudo-nitzschia calliantha</i> Lundholm, Moestrup et Hasle (bacillariophyta) isolated from the Black Sea

Besiktepe S., Ryabushko L., Ediger D., Yimaz D., ZENGİNER YILMAZ A., Ryabushko V., ...Daha Fazla

HARMFUL ALGAE, sa.4, ss.438-442, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.hal.2007.09.004
  • Dergi Adı: HARMFUL ALGAE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.438-442
  • Anahtar Kelimeler: domoic acid, harmful algae, diatom, Pseudo-nitzschia calliantha, Black Sea, PECTEN-MAXIMUS, KING SCALLOP, PHYTOPLANKTON, PSEUDODELICATISSIMA, MULTISERIES, CONFIRMATION, SHELLFISH, MORTALITY, PHOSPHATE, PUNGENS
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

A species of Pseudo-nitzschia isolated from Sevastopol Bay, Black Sea, was examined for its toxicity. The species was identified as P. calliantha Lundholm, Moestrup et Hasle based on SEM and TEM examination. Domoic acid (DA) was detected in batch culture throughout the growth cycle of P. calliantha. The production of DA by this diatom species was confirmed by fluorenylmethoxycarbonyl (FMOC) derivatization and HPLC-fluorescence method. The cellular DA level was higher in the early exponential phase, with the maximum value of 0.95 pg DA cell(-1). In the stationary phase, the cellular DA levels declined. This is the first record of a DA producing diatom isolated from the Black Sea. (C) 2007 Elsevier B.V. All rights reserved.