Collagen-chondroitin sulfate-based PLLA-SAIB-coated rhBMP-2 delivery system for bone repair

Keskin D., Tezcaner A., KORKUSUZ P., KORKUSUZ F., HASIRCI V. N.

BIOMATERIALS, cilt.26, sa.18, ss.4023-4034, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 18
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.biomaterials.2004.09.063
  • Dergi Adı: BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4023-4034
  • Anahtar Kelimeler: collagen, chondroitin sulfate, PLLA, SAIB, rhBMP-2, controlled drug delivery, MORPHOGENETIC PROTEIN-2, CONTROLLED-RELEASE, BIOMATERIAL CARRIERS, PHARMACOKINETICS, COMPOSITE, DEFECTS, POLYMER, SPONGE
  • Orta Doğu Teknik Üniversitesi Adresli: Evet

Özet

Bone morphogenetic proteins (BMPs) are osteoinductive proteins used intensively in clinical investigations involving various bone-related treatments. Owing to their high potential in new bone formation they require local application at the treatment site. For this purpose various controlled delivery systems with BMPs as the excipients have been prepared in recent years. Focusing on this clinical need a disc-shaped BMP carrier was designed as a local delivery system using soluble collagen and chondroitin sulfate. In situ release studies carried out with a model protein (FITC-labeled Protein A) presented a very high rate of release; with most of the protein content being released within 24h. This rate could be decreased by providing a poly(L-lactide) (PLLA) and sucrose acetate isobutyrate-based (SAIB-based) coat around the release system, applied after BMP loading. In this way, it was possible to extend the release period from 24 h to about 12 days. In situ release of BMP from the same carriers, as quantitated using an ELISA kit, was even slower, with 50% of the protein being released in 15 days.