GLUCOSE-OXIDASE IMMOBILIZED ON GELATIN BY VARIOUS CROSS-LINKERS


ELCIN Y., SUNGUR S., AKBULUT U.

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, ss.137-147, 1993 (SCI İndekslerine Giren Dergi) identifier

  • Basım Tarihi: 1993
  • Dergi Adı: JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
  • Sayfa Sayıları: ss.137-147

Özet

Immobilization of glucose oxidase have been realized with photographic gelatin as support for chemical coupling. Chromium acetate, potassium chromium sulfate and formaldehyde were used as crosslinking agents to immobilize glucose oxidase on film strips. Enzyme, crosslinker concentrations, temperature, pH and reuse number on native and immobilized glucose oxidase activity and water contents of unused and used films were investigated. The best results were achieved with the crosslinker chromium acetate as 50% maximum activity after 40 assays.