GLUCOSE-OXIDASE IMMOBILIZED ON GELATIN BY VARIOUS CROSS-LINKERS
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, pp.137-147, 1993 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Publication Date: 1993
- Journal Name: JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.137-147
- Middle East Technical University Affiliated: Yes
Abstract
Immobilization of glucose oxidase have been realized with photographic gelatin as support for chemical coupling. Chromium acetate, potassium chromium sulfate and formaldehyde were used as crosslinking agents to immobilize glucose oxidase on film strips. Enzyme, crosslinker concentrations, temperature, pH and reuse number on native and immobilized glucose oxidase activity and water contents of unused and used films were investigated. The best results were achieved with the crosslinker chromium acetate as 50% maximum activity after 40 assays.