Application of novel Fe3O4-g-GO-g-RAFT agent nanoabsorbents for D-SPME of biogenic amines in smoked fish


Molaei R., Tajik H., Moradi M., Forough M.

Journal of Food Composition and Analysis, vol.87, 2020 (Journal Indexed in SCI Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 87
  • Publication Date: 2020
  • Doi Number: 10.1016/j.jfca.2019.103400
  • Title of Journal : Journal of Food Composition and Analysis
  • Keywords: Biogenic amine, HPLC, Magnetic, Graphene oxide, RAFT agent, Smoked fish, SOLID-PHASE EXTRACTION, PERFORMANCE LIQUID-CHROMATOGRAPHY, MOLECULARLY IMPRINTED POLYMERS, MAGNETIC GRAPHENE OXIDE, LACTIC-ACID BACTERIA, GAS-CHROMATOGRAPHY, RAFT POLYMERIZATION, MASS-SPECTROMETRY, MICROEXTRACTION, NANOPARTICLES

Abstract

© 2019 Elsevier Inc.Herein, a dispersive solid phase microextraction (D-SPME) approach based on magnetic nanoabsorbents was developed for the simultaneous extraction of histamine, cadaverine, putrescine, and tyramine in smoked fish. The desired magnetic nanocomposite was prepared with surface modification of Fe3O4 nanoparticles with graphene oxide via surface reversible addition fragmentation chain transfer (RAFT) copolymerization. Preparation, size, morphology, surface functional groups and magnetic properties of as-synthesized nanocomposite was characterized. The obtained results showed acceptable r2 values (≥0.9949) for all analyte as well as good recovery (>98.36), repeatability (RSD%<3.57), and reproducibility (RSD%<5.16) of the developed method. The developed method was successfully employed to estimate four biogenic amines in 40 samples from different species of smoked fishes available in northern Iran. Thirty-six out of 40 samples exceeded an acceptable level of histamine (50 mg Kg−1). Cadaverine and putrescine were founded at a higher level, while tyramine was detected at the lowest level in comparison with other BAs (<11 %).